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2-Pyrrolidinone (Synonyms: 2-吡咯烷酮; 2-吡咯酮; 2-Pyrrolidone) 纯度: ≥98.0%
2-Pyrrolidinone 是存在于甘蓝中的活性化合物,并显示抗癌特性。
2-Pyrrolidinone Chemical Structure
CAS No. : 616-45-5
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1 g | ¥800 | In-stock | |
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10 g | 询价 |
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生物活性 |
2-Pyrrolidinone is an active compound present in Brassica oleracea var. capitata and displays anticancer property. |
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体外研究 (In Vitro) |
Radical scavenging activity increases with increasing the amount of 2-Pyrrolidinone. The cytotoxicity of 2-Pyrrolidinone is found to be dose and time dependent, and the effect is observed microscopically in all of the selected cancer cell lines. It is observed that 2-Pyrrolidinone has cytotoxic concentrations of 2.5 mg/mL for HeLa, 3 mg/mL for PC-3 cells at 24 h and 1.5 mg/mL for HeLa and 2 mg/mL for PC-3 cells at 48 h, respectively. The cell viability decreases with increasing concentrations of purified 2-Pyrrolidinone. After treatment with 2-Pyrrolidinone their IC50 concentrations (1.5 mg/mL and 2 mg/mL for HeLa and PC-3 cells, respectively) for a period of 24 h, morphological changes in the cells are observed. It is demonstrated that 2-Pyrrolidinone induces apoptosis in HeLa and PC-3 cells via G0/G1phase of cell cycle arrest[1]. Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
85.10 |
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Formula |
C4H7NO |
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CAS 号 |
616-45-5 |
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性状 |
固体 |
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颜色 |
White to off-white |
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中文名称 |
2-吡咯烷酮 |
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结构分类 |
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初始来源 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:
H2O 中的溶解度 : 100 mg/mL (1175.09 mM; 超声助溶) 配制储备液
查看完整储备液配制表
* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2
动物溶解方案计算器
请输入动物实验的基本信息:
给药剂量 mg/kg 动物的平均体重 g 每只动物的给药体积 μL 动物数量 只 由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
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E-mail:sales@jinpanbio.com 技术支持电话:021-50837765 技术支持邮箱:sales@jinpanbio.com |
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纯度 & 产品资料 |
纯度: ≥98.0%
Data Sheet (599 KB) SDS (418 KB)
COA (195 KB) HNMR (266 KB) 产品使用指南 (1538 KB) |
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参考文献 |
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Cell Assay [1] |
The inhibitory concentrations (IC50) are evaluated using an MTT assay. Cancer cells are grown (1×104 cells/well) in a 96-well plate for 48 h into 75% confluence. The medium is replaced with fresh medium containing concentration of (0.5 to 5 mg/mL) 2-Pyrrolidinone, and the cells are further incubated for 24 h and 48 h. The culture medium is removed, and 100 mL of the MTT solution is added to each well and incubated at 37°C for 4 h. After removal of the supernatant, 50 mL of DMSO is added to each of the wells and incubated for 10 min to solubilize the formazan crystals. The optical density is measured at 620 nm in an ELISA multi-well plate reader. The OD value is used to calculate the percentage of viability[1]. Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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