标签归档：lnnt

Lacto-N-neotetraose (Synonyms: LNnT)

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Lacto-N-neotetraose  (Synonyms: LNnT) 纯度: 99.71%

Lacto-N-neotetraose (LNnT) 是一种内源性代谢物 (endogenous metabolite)。Lacto-N-neotetraose 可以抑制 TNF-α 诱导的未成熟上皮细胞中的 IL-8 分泌。Lacto-N-neotetraose 具有抗炎活性，可提高伤口愈合能力。

Lacto-N-neotetraose (Synonyms: LNnT)

Lacto-N-neotetraose Chemical Structure

CAS No. : 13007-32-4

规格 价格 是否有货 数量
1 mg ￥2200 In-stock
5 mg ￥5500 In-stock
10 mg ￥8900 In-stock
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100 mg   询价  

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Lacto-N-neotetraose 相关产品

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生物活性

Lacto-N-neotetraose (LNnT) is an endogenous metabolite. Lacto-N-neotetraose can inhibit TNF-α induced IL-8 secretion in immature epithelial cells. Lacto-N-neotetraose has anti-inflammatory avtivity, and can improve the wound closure[1][2][4].

IC50 & Target

Endogenous Metabolite[1], TNFR1[2].
体外研究
(In Vitro)

Lacto-N-neotetraose (5 mg/mL, 24 h) induces IL-8 secretion in T84 cells[2].
Lacto-N-neotetraose (5 mg/mL, 24 h) reduces TNF-α induced IL-8-secretion with 38% in FHs 74 Int cells[2].
Lacto-N-neotetraose binds TNFR1 (Tumor necrosis factor receptor 1) with a Kd value of 900 ± 660 nM[2].
Lacto-N-neotetraose (5 mg/mL, 24 h) attenuates TNF-α induced inflammation by TNFR1 ectodomain shedding in FHs 74 Int cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Lacto-N-neotetraose 相关抗体:
体内研究
(In Vivo)

Lacto-N-neotetraose (100 μM, intratracheal administration for 24 h) reduces the abundance of Streptococcus pneumonia in the lungs of pneumonia rabbits[3].
Lacto-N-neotetraose (100/200 μg, Intradermal injection, at 3, 7, 14, and 21 days post-surgery) increases the wound closure rate on day 7 post-wounding[4].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rabbit model of pneumonia[3]
Dosage: 100 μM
Administration: Intratracheal administration for 24 h
Result: Decreased by ~2 logs the bacterial load in the lung at 48 h after challenge.
Eliminated the extensive, edematous right middle lobe lesion evident at 48 h in control animals.
Animal Model: Mice with symmetric full-thickness wounds
Dosage: 100, 200 μg
Administration: Intradermal injection, at 3, 7, 14, and 21 days post-surgery.
Result: Exhibited better healing score, follicle formation, and lower epidermal thickness index (H&E staining).

分子量

707.63

Formula

C26H45NO21
CAS 号

13007-32-4
性状

固体

颜色

White to off-white

结构分类
  • Saccharides
  • Polysaccharides
  • Others
初始来源
  • 内源性代谢物

human
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

H2O 中的溶解度 : 125 mg/mL (176.65 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4132 mL 7.0658 mL 14.1317 mL
5 mM 0.2826 mL 1.4132 mL 2.8263 mL
10 mM 0.1413 mL 0.7066 mL 1.4132 mL

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* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

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体积 (start)

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动物溶解方案

动物溶解方案计算器
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给药剂量

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该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
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纯度 & 产品资料

纯度: 99.71%

Data Sheet (613 KB) SDS (251 KB)

COA (184 KB) HNMR (304 KB) RP-HPLC (216 KB) MS (70 KB)

产品使用指南 (1538 KB)

参考文献

  • [1]. Xiaomin Dong, et al. CRISPRi-Guided Multiplexed Fine-Tuning of Metabolic Flux for Enhanced Lacto- N-neotetraose Production in Bacillus subtilis. J Agric Food Chem. 2020 Feb 26;68(8):2477-2484.  [Content Brief]

    [2]. Lianghui Cheng, et al. The Human Milk Oligosaccharides 3-FL, Lacto-N-Neotetraose, and LDFT Attenuate Tumor Necrosis Factor-α Induced Inflammation in Fetal Intestinal Epithelial Cells In Vitro through Shedding or Interacting with Tumor Necrosis Factor Rece  [Content Brief]

    [3]. I Idänpään-Heikkilä, et al. Oligosaccharides interfere with the establishment and progression of experimental pneumococcal pneumonia. J Infect Dis. 1997 Sep;176(3):704-12.  [Content Brief]

    [4]. Behrouz Farhadihosseinabadi, et al. The in vivo effect of Lacto-N-neotetraose (LNnT) on the expression of type 2 immune response involved genes in the wound healing process. Sci Rep. 2020 Jan 22;10(1):997.  [Content Brief]

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