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Cyclic-di-GMP sodium (Synonyms: c-di-GMP sodium; cyclic diguanylate sodium; 5GP-5GP sodium)

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Cyclic-di-GMP sodium  (Synonyms: c-di-GMP sodium; cyclic diguanylate sodium; 5GP-5GP sodium)

Cyclic-di-GMP sodium 是一种 STING 激动剂，也是细菌第二信使，协调细菌生长和行为的不同方面，包括运动能力、毒力、生物膜的形成和细胞周期的进展。Cyclic-di-GMP sodium 具有抗癌细胞增殖活性，还可诱导 CD4 受体表达升高和细胞周期停滞。Cyclic-di-GMP sodium 可用于癌症的研究。

Cyclic-di-GMP sodium (Synonyms: c-di-GMP sodium; cyclic diguanylate sodium; 5GP-5GP sodium)

Cyclic-di-GMP sodium Chemical Structure

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Cyclic-di-GMP sodium 的其他形式现货产品:

Cyclic-di-GMP diammonium Cyclic-di-GMP disodium

Cyclic-di-GMP sodium 相关产品

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生物活性

Cyclic-di-GMP sodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP sodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP sodium can be used in cancer research[1][2][3][4].

IC50 & Target

STING[1][2][3][4].
体外研究
(In Vitro)

Cyclic-di-GMP sodium (0.5-50 µM; 5 days) inhibits proliferation of human colon cancer cells[1].
Cyclic-di-GMP sodium (0.5-50 µM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
Cyclic-di-GMP sodium (0.5-50 µM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclic-di-GMP sodium 相关抗体:

Cell Proliferation Assay[1]

Cell Line: H508 cells
Concentration: 0.5-50 µM
Incubation Time: 5 days
Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation.

Cell Viability Assay[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: Jurkat cells
Concentration: 50 µM
Incubation Time: 24 h
Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.

体内研究
(In Vivo)

Cyclic-di-GMP sodium (100 µg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (B6) mice (8- to 10-week-old)[3].
Dosage: 100 µg/per
Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax.
Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax.

Formula

C20H24N10NaO14P2
结构分类
  • Ketones, Aldehydes, Acids
初始来源
  • 微生物
  • 内源性代谢物

bacterial species
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
Data Sheet (545 KB) 产品使用指南 (1538 KB)

参考文献

  • [1]. Karaolis DK, et al. 3′,5′-Cyclic diguanylic acid (c-di-GMP) inhibits basal and growth factor-stimulated human colon cancer cell proliferation. Biochem Biophys Res Commun. 2005 Apr 1;329(1):40-5.  [Content Brief]

    [2]. Steinberger O, et al. Elevated expression of the CD4 receptor and cell cycle arrest are induced in Jurkat cells by treatment with the novel cyclic dinucleotide 3′,5′-cyclic diguanylic acid. FEBS Lett. 1999 Feb 5;444(1):125-9.  [Content Brief]

    [3]. Wang Z, et al. STING activator c-di-GMP enhances the anti-tumor effects of peptide vaccines in melanoma-bearing mice. Cancer Immunol Immunother. 2015 Aug;64(8):1057-66.  [Content Brief]

    [4]. Jenal U, et al. Cyclic di-GMP: second messenger extraordinaire. Nat Rev Microbiol. 2017 May;15(5):271-284.  [Content Brief]

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