标签归档：Megazyme酶试剂

爱尔兰Megazyme酶 Enzymes

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爱尔兰Megazyme酶 Enzymes

       爱尔兰Megazyme是一家全球领先的制造和供应高品质和创新的检测谷物，食品，饲料，发酵，乳制品和葡萄酒行业的技术。Megazyme成立于1988年，该公司目前提供了超过70种诊断试剂盒和超过300种其他试剂和底物。

该公司产品分以下系列：

1. 生物及食品酶法分析试剂盒 (Diagnostic Kits)
2. 试剂混合物(Reagent Mixtures)
3. 协同因子(Cofactors)
4. 糖酶片剂试验(Carbohydrase Tablet Tests)
5. 蛋白酶片剂试验(Protease Test Tablets)
6. 可溶性呈色底物(Soluble Chromogenic Substrates)
7. 不可溶性（交联的）呈色底物(Insoluble (Crosslinked) Chromogenic Substrates)
8. 酶(Enzymes)
9. 植物凝血素(Lectins)
10. 多聚糖(Polysaccharides)
11. 低聚糖(Oligosaccharides)
12. 相关产品 (General)

 英文名称  酶中文名 规格（酶活性Units） 货号
Acetate Kinase(E.coli) 醋酸激酶[大肠杆菌] 100000 E-ACKEC
RecAcetyl-CoA ynthetase (B.subtilis) Acetyl-CoA合成酶[枯草杆菌] 250 E-ACSBS
Adenylate Kinase (Myokinase)(prokaryote) 转化腺苷酸激酶[肌激酶][原核生物] 10000 E-AMPK
Alcohol dehydrogenase(E.coli) 乙醇脱氢酶[大肠杆菌] 1000 E-ADHEC
Alpha-Amylase(A.oryzae) Alpha淀粉酶[米麴菌] 20000 E-ANAAM
Alpha-Amylase(B.licheniformis) Alpha淀粉酶[苔藓杆菌] 120000 E-BLAM
β-Amylase(Barley) Beta淀粉酶[大麦] 100000 E-BARBL
β-Amylase(Barley) Beta淀粉酶[大麦] 2g E-BARBP
β-Amylase(B.cereus) Beta淀粉酶[仙人掌杆菌] 100,000 E-BCBAM
Amyloglucosidase(A.niger) 淀粉葡萄糖苷酶[黑曲霉] 140,000 E-AMGDF
Amyloglucosidase(Rhizopus sp.) 淀粉葡萄糖苷酶[根霉] 5,000 E-AMGPU
endo-1,5-α-L-Arabinanase(A.niger) 1,5-α-L-阿拉伯糖内切酶[黑曲霉] 140 E-EARAB
α-L-Arabinofuranosidase(A.niger)) α-L-阿拉伯呋喃糖酶[黑曲霉] 300 E-AFASE
Recα-L-Arabinofuranosidase(Novel Specificity) α-L-阿拉伯呋喃糖酶[新] 400 E-AFAM2
Alpha-aspartyl dipeptiease(E.coli) Alpha-天冬氨酰二肽酶[大肠杆菌] 2,000 E-DIPEP
Celobiohydrolase I(T.longibrachiatum) 纤维素二糖水解酶I[长枝木霉] 20mg E-CBHI
Cellulase(endo-1,4-D-Glucanase)(T.longibrachiatum) 纤维素酶[1,4-D-葡聚糖内切酶[长枝木霉] 1,000 E-CELTR
Cellulase(endo-1,4-D-Glucanase)(T.emersonii) 纤维素酶[1,4-D-葡聚糖内切酶[Emersonii蓝状菌] 2,000 E-CELTE
Celluase(endo-1,4-D-glucanse)(A.niger) 纤维素酶[1,4-D-葡聚糖内切酶[黑曲霉] 2,000 E-CELAN
Reccitrate synthase(E.coli) 柠檬酸合酶[大肠杆菌] 50 E-CITEC
Cytidylate Kinase(Prokaryote) 胞嘧啶核苷酸激酶[原核生物] 500 E-CMPK
RecDiaphorase(D.coli) 黄递酶[大肠杆菌] 1,000 E-DIAEC
Formate dehydrogenase(C.boidini) 甲酸脱氢酶[C.boidini] 300 E-FDHCB
CructanaseMixture(Purified) 果糖酶混合物[已纯化] 40ml E-FRMXLQ
Fructanase Mixture Powder 果糖酶混合物 40,000 E-FRMXPD
endo-1,4-β-D-Galactanase(A.niger) 1,4-β-D-半乳糖内切酶[黑曲霉] 1,000 E-EGALN
RecGalactose Dehydrogenase(soli prokaryote) 半乳糖脱氢酶[土壤原核生物] 200 E-GALDH
α-Galactosidase(A.niger) α半乳糖苷酶[黑曲霉] 2,000 E-AGKAN
α-Galactosidase(G.uar) α半乳糖苷酶[瓜尔豆] 600 E-AGLGU
β-Galactosidase(A.niger) β半乳糖苷酶[黑曲霉] 8,000 E-BGLAN
β-Galactosidase(Lactase)(K.fragilis) β半乳糖苷酶[乳糖酶][脆壁克鲁维酵母] 4,000 E-LACTS
endo-1,3-β-Glucanase(Trichoderma.sp.) 1,3-β-D葡聚糖内切酶[木霉菌] 100 E-LAMSE
exo-1,3-β-Glucanase(Trichoderma.sp.) 1,3-D-β-葡聚糖外切酶[木霉菌] 400 E-EXBGL
Gluconokinase(E.coli) 葡糖酸激酶[大肠杆菌] 1,500 E-GLUKEC
Glucose 6-phosphate dehydrogenase 葡萄糖6磷酸脱氢酶 5,000 E-GPDH5
Glucose 6-phosphate dehydrogenase 葡萄糖6磷酸脱氢酶 25,000 E-GPDH25
a-Glucosidase(Maltase)(yeast) Alpha葡[萄]糖苷酶[麦芽糖酶][酵母] 2,000 E-MALTS
a-Glucosidase(B.stearothermophilus) Alpha葡[萄]糖苷酶[嗜热脂肪芽孢杆菌] 1,500 E-TSAGL
a-Glucosidase(Transglucosidase)(A.niger) Alpha葡[萄]糖苷酶[转葡萄糖苷酶][黑曲霉] 2,000 E-TRNGL
β-Glucosidase(A.niger) Beta葡[萄]糖苷酶[黑曲霉] 200 E-BGLUC
Recβ-Glucosidase(Agrobacterium sp.) Beta葡[萄]糖苷酶[农杆菌] 600 E-BGISAG
Recβ-Glucosidase(thermostable)(T.maritima) Beta葡[萄]糖苷酶[耐热][热海栖热孢菌] 230 E-BGOSTM
Glutamate dehydrogenase(E.coli) 谷氨酸脱氢酶[大肠杆菌] 10,000 E-GLDHEC
RecGlutamate Oxaloccetate Transaminase(E.coli) 谷氨酸草酰乙酸转氨酶[大肠杆菌] 5,000 E-GOTEC
Glutamate pyruvate transminase(B.subtilils) 谷氨酸丙酮酸转氨酶[枯草杆菌] 2,500 E-GPTBS
Glucose Oxidase/catalase Mixture 葡萄糖氧化酶/催化酶混合物 2 vials E-GOXCA
Guanylate Kinase(prokayote) 鸟苷酸激酶[原核生物] 500 E-GMPK
3-Hydrxybutyrate dehydrogenase(prokaryote) 3-羟丁酸脱氢酶[原核生物] 200 E-HBDH
Hexokinase 已糖激酶 10,000 E-HEX10
Hexokinase 已糖激酶 50,000 E-HEX50
Hexokinase(420U/ml)+G6PDH(210U/ml) 已糖激酶[420U/ml]+葡萄糖-6-磷酸脱氢酶[210U/ml] 10ml E-HKGDH
RecHaluronate lyase(novel specificity)(soil prokaryote) 透明质酸[裂解]酶[新品][土壤原核生物] 50 E-HYLSP
endo-Inulinase(A.niger) 菊粉内切酶[黑曲霉] 200 E-ENDOI
exo-Inulinase(A.niger) 菊粉外切酶[黑曲霉] 5000 E-EXOI
Invertase(Fructofuranosidase)(yeast) 蔗糖酶[呋喃果糖苷酶][酵母] 150000 E-INVRT
Isoamylase(Glycogen 6-glucanohydrolase) 异淀粉酶[糖原6-葡萄糖苷酶] 500 E-ISMY
Isocitrate dehydrogenase (B.subtilis) 异柠檬酸脱氢酶[枯草杆菌] 2000 E-ICDHBS
Lichenase(endo-1,3(4)-D-Glucanase)(Bacillussp.) 地衣聚糖酶[1,3(4)-D-葡聚糖内切酶[芽孢杆菌] 5000 E-LICHN
RecD-Malate dehydrogenase(E.coli) D-苹果酸脱氢酶[大肠杆菌] 200 E-DMDHEC
RecL-Malate dehydrogenase(E.coli) L-苹果酸脱氢酶[大肠杆菌] 50000 E-LMDHEC
endo-1,4-β-Mannanase(A.niger)) 1,4-β甘露聚糖内切酶[黑曲霉] 500 E-BMANN
endo-1,4-β-Mannanase(Bacillus sp.) 1,4-β甘露聚糖内切酶[芽孢杆菌] 2000 E-BMABS
RecMannitol dehydrogenase(P.fluorescens) 甘露醇脱氢酶[荧光假单胞菌] 500 E-MNHPF
Rec-β-Mannosidase(C.fimi) β甘露糖苷酶[纤维单胞菌] 200 E-BMOSCF
Pectte Lyase M1(Aspergillus sp.) 果胶酸裂合酶M1[曲霉属] 600 E-PECLY
RecPectate Lyase M2(C.japonicus) 果胶酸裂合酶M2[日本龟蜡蚧] 2500 E-PLYCJ
RecPhosphoglucose Isomerse (B.subtillis) 磷酸葡糖异构酶[枯草杆菌] 5000 E-PGIBS
RecPhosphoglucose Isomerse (B.subtillis) 磷酸葡糖异构酶[枯草杆菌] 50000 E-PGIBSB
RecPhosphoglucose Isomerse (E.coli) 磷酸葡糖异构酶[大肠杆菌] 10000 E-PGIEC
RecPhosphoglucose Isomerse (E.coli) 磷酸葡糖异构酶[大肠杆菌] 50000 E-PGIECB
6-Phosphogluconate dehydrogenase(E.coli) 6-磷酸葡糖酸脱氢酶[大肠杆菌] 150 E-PGDHEC
RecPhosphoglucose Isomerse (S.cerevisiae) 磷酸葡糖异构酶[酿酒酵母] 5000 E-PGISC
RecPhosphoglucose Isomerse (S.cerevisiae) 磷酸葡糖异构酶[酿酒酵母] 50000 E-PGISCB
RecPhosphoglucose Isomerse (E.coli) 磷酸甘露糖异构酶[大肠杆菌] 1000 E-PMIEC
endo-Polygalacturonanase M1(A.niger) 聚半乳糖醛酸M1[黑曲霉] 5000 E-PGALS
endo-Polygalacturonanase M2(A.niger) 聚半乳糖醛酸M2[黑曲霉] 10000 E-PGALUSP
Protease(Subtillisin A)(B.licheniformis) 蛋白酶[枯草杆菌][对地衣芽孢杆菌] 2 g(40ml) E-BSPRT
Pullulanase M1(K.planticola) 普鲁兰酶M1[植生克雷伯氏菌] 2000 E-PULKP
Pullulanase M2(B.licheniformis) 普鲁兰酶M2[对地衣芽孢杆菌] 2000 E-PULBL
Pullulanase M3(B.acidopullulyticus) 普鲁兰酶M3[嗜酸普鲁兰芽孢杆菌] 5000 E-PULBA
Succinyl-CoA synthetase(prokaryote) 琥珀酰辅酶A合成酶[原核生物] 550 E-SCOAAS
Sucrase (Maltase)(yeast) 蔗糖酶[麦芽糖酶][酵母] 220 E-SUCR
Thymidylate Kinase (prokaryote) 胸苷酸激酶[原核生物] 150 E-TMPK
Xylanase M1(T.viride) 木聚糖酶M1[绿色木霉菌] 8000 E-XYTR1
Xylanase M2(T.longibrachiatum;pl 5.5) 木聚糖酶M2[长枝木霉] 4000 E-XYTR2
Xylanase M3(T.longibrachiatum;pl 9.0) 木聚糖酶M3[长枝木霉] 8000 E-XYTR3
Xylanase M4(A.niger) 木聚糖酶M4[黑曲霉] 8000 E-XYTR4
Xylanase M6(rumen microorganism) 木聚糖酶M6[瘤胃微生物] 8000 E-XYRU6
Alkaline phosphatase(E.coli) 碱性磷酸酶(大肠杆菌) 400 E-ALPEC
Glutaminase(E.coli) 谷氨酰胺酶(大肠杆菌) 2500 E-GLUTEC
Guanylate Kinase(prokayote) 鸟[嘌呤核]苷酸激酶(原核生物) 500 E-GMPK
3-Hydrxybutyrate dehydrogenase(prokaryote) 3-羟基苯甲酸酯脱氢酶(原核生物) 200 E-HBDH
myo-Inositol dehydrogenase(B.subtillis) 肌环己六氢醇脱氢酶(枯草芽孢杆菌) 500 E-INDH
D-Lactate dehydrogenase(L.mesenteroides) D-乳酸盐脱氢酶(肠膜明串珠菌) 15000 E-DLDHLM
β-Mannanase(T.maritima) Beta甘露聚糖酶(海栖热袍菌) 250 E-BMATM
Malt Amylase Standard 麦芽淀粉酶标准液 100ml E-MAST

 

纤维素酶检测试剂盒

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纤维素酶检测试剂盒[CellG5法], Cellulase Assay Kit (CellG5 Method)

货号：K-CellG5
中文品名：纤维素酶检测试剂盒[CellG5法]
品名：Cellulase Assay Kit (CellG5 Method)
规格1：K-CellG5-4V 120/240次(手工)/480次(自动分析仪)
规格2：K-CellG5-2V 60/120次(手工)/240次(自动分析仪)
说明：CellG5检测试剂盒用于检测纤维素内切酶（1,4 -β-葡聚糖内切酶）包含两种组分：1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) 和 2)热稳定的β-葡萄糖苷酶。 酮阻断基团可阻止β-葡萄糖苷酶对BPNPG5的水解。通过纤维素内切酶的培养可生成非阻断的显色寡糖，被β-葡萄糖苷酶迅速水解。产生4-硝基苯酚的速率与β-葡萄糖苷酶水解BPNPG5直接相关。加入Tris缓冲液(pH 9.0)后可终止反应，并形成苯酚显色产物。
CellG5检测法代表了代表了纤维素酶测量方法的巨大进步。传统的纤维素酶检测依赖于酶底物，例如羧甲基纤维素，微晶纤维素，纤维低聚糖，CMC滤纸，染色多糖，包括CMC刚果红或纤维素天青。
应用：纤维素酶检测试剂盒[CellG5法]。

原理：
比色法测定酶制剂、发酵产品中1,4 -β-葡聚糖内切酶（纤维素酶）的含量。
纤维素酶检测试剂盒 （K-CellG5）检测原理
检测方法：分光光度法 @400 nm
反应时间：10min
检测限：3.5 x 10-4 U/mL
应用案例：
发酵液，工业酶制剂和生物燃料研究。
方法识别：
新方法
试剂盒组成：
Bottle 1: (x2) 或 (x4) 每瓶含有4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5)溶于3毫升含有0.02% w/v叠氮钠的10% DMSO水溶液。
-20°C下稳定超过4年。
Bottle 2: (x 2) 热稳定的β-葡萄糖苷酶(0.50 mL, 600 U/mL)溶于含有0.02% w/v叠氮钠的 50% w/v 硫酸铵溶液。
4°C下稳定超过4年。
Bottle 3: 木霉纤维素酶标准溶液 (5 mL, ~ 3 U/mL; 准确至见瓶标签) 溶于含有0.02% w/v叠氮钠的50% 的丙三醇水溶液。
-20°C下稳定超过4年。
优点：

所有试剂可稳定超过4年
价格非常具有竞争力
完全针对纤维素酶（1,4-葡聚糖内切酶)
常规操作，非常灵敏
检测方法十分简单，适合仪器分析
包含标准
参考文献：
Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase). McCleary, B. V., Mangan, D., Daly, R., Fort, S., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 385, 9-17.
Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase. Mangan, D., McCleary, B. V., Liadova, A., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 395, 47-51.
A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase). Mangan, D., Cornaggia, C., McKie, V., Kargelis. T. & V. McCleary, B. V. (2016). Analytical and Bioanalytical Chemistry, 408(15), 4159-4168.
说明书下载地址:https://secure.megazyme.com/files/Booklet/K-CellG5_DATA.pdf

内切纤维素酶检测试剂盒

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内切纤维素酶检测试剂盒

英文名：Cellulase Assay Kit (CELLG3 Method)
货号：K-CELLG3
规格：180 / 360 assays per kit / 720 (auto-analyser)
市场价： 5537元

 纤维素酶是一种重要的酶产品，是一种复合酶，主要由外切β-葡聚糖酶、内切β-葡聚糖酶和β-葡萄糖苷酶等组成，还有很高活力的木聚糖酶。
提供内切纤维素酶检测试剂盒，色度法（400 nm）测定酶制品和发酵产品中的纤维素酶（内切-1,4-β-葡聚糖酶）。
内切纤维素酶检测试剂盒(CELLG3方法) K-CELLG3
使用高纯度β-葡萄糖苷酶和苯亚甲基阻断，2 – 氯-4 – 硝基苯基-β-Dcellotrioside（BClPNPβ-G3）。 β-葡萄糖苷酶的能力确保测定的可靠的最大的灵敏度。BClPNPβ-G3的水解为苯亚甲基，通过纤维素酶阻断纤维二糖和2-氯-4 -硝基苯基-β-D-葡萄糖， 2-Cl-4-硝基苯基-β-D-葡萄糖通过β-葡萄糖苷酶立即裂解为D-葡萄糖和游离的2 – 氯-4 – 硝基苯酚（ClPNP）。
反应时间：16min
适用样品：酶制品和发酵产品
优点：
价格低廉（每次检测成本）
特异性高
方法简单
包含标准品
 
The CELLG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;

1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase.  The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase.  The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CELLG5) is now available for the measurement of endo-cellulase.  The CELLG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases.  In addition, the exchange of the benzylidene blocking group in CELLG3 for 3-keto-butylidene in CELLG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay.  As DMSO is known to inhibit certain cellulases, this is another benefit in using CELLG5.  Megazyme now recommends the use of K-CELLG5 for all assays for the measurement of endo-cellulase.
Colourimetric method for the determination of 
endo-1,4-β-glucanase (cellulase) in enzyme preparations and fermentationproducts
Principle:
(endo-1,4-β-glucanase)
(1) 3-Ketobutylidene-G5-β-PNP + H2O → Blocked-GX + G(5-X)-β-PNP
(thermostable β-glucosidase)
(2) G(5-X)-β-PNP + H2O → D-glucose + PNP
(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-CELLG5-4V 120 / 240 assays (manual) / 480 (auto-analyser)
or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)
Method:                         Spectrophotometric at 400 nm
Total assay time:           10 min
Detection limit:                3.5 x 10-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition:     Novel method

 

Beta葡聚糖[混联]检测试剂盒

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β-葡聚糖[混联]检测试剂盒
产品货号：K-BGLU
产品品名：β-葡聚糖[混联]检测试剂盒
英文品名：β-Glucan (Mixed Linkage) Assay Kit
规格型号：100 assays per kit
β-葡聚糖是用独特的工艺开发的一种新的产品，其来源于新鲜的食品啤酒酵母。它是一种多糖，主要化学结构β-1，3 葡聚糖和β-1，6葡聚糖，其中前者具有抗肿瘤性质，而且能够极大地提高人体自然免疫力。
上海金畔生物科技有限公司提供β-葡聚糖[混联]检测试剂盒，比色法（510nm）测定谷物、饲料、食品、饮料和其他原料中的B-葡聚糖。
原理：
总检测时间：100min
检测限：样品重量的0.5-100%
适用样品：燕麦、大麦、麦芽、麦芽汁、啤酒、食品以及其他原料
优点：
成本低
提供的所有试剂稳定性>2年
只有酶试剂盒可用
特异性高
方法简单
包含标准品
方法认证：AOAC (Method 995. 16)、AACC (Method 32-23),EBC (Methods 3.11.1,8.16.l 和 8.11. 1)  ICC(标准号l66)，and RACI（标准方法）

分析物意义： 大麦和燕麦的主要细胞壁多糖 
Megazyme检测试剂盒优点：反应迅速、试剂稳定， 只有酶检测试剂盒可用。 AOAC方法 995.16; AACC 方法 32-23
AACC Method 32-23.01, AOAC Method 995.16, EBC Methods 3.11.1, 4.16.1 and 8.11.1, ICC Standard No. 166 and RACI standard method for the measurement of 1,3:1,4-ß-D-glucan in cereal grains, milling fractions, wort and beer. Recommended/Standard procedure of the Association of Official Analytical Chemists (AOAC), American Association of Cereal Chemists (AACC), Royal Australian Chemical Institute (RACI), European Brewing Convention (EBC), and International Association for Cereal Science and Technology (ICC). Content:100 assays per kit
Colourimetric method for the determination of β-Glucan in
cereal grains, feed, foodstuffs, beverages and other materials
Principle:
(lichenase)
(1) β-Glucan + H2O → β-gluco-oligosaccharides
(β-glucosidase)
(2) β-Gluco-oligosaccharides + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 100 min
Detection limit: 0.5-100% of sample weight
Application examples:
Oats, barley, malt, wort, beer, food and other materials
Method recognition:
AOAC (Method 995.16), AACC (Method 32-23.01), EBC (Methods
3.10.1, 4.16.1 and 8.13.1), ICC (Standard No. 166), RACI (Standard
Method) and CODEX (Type II Method)
Advantages

  • Very cost effective
  • All reagents stable for > 2 years as supplied
  • Only enzymatic kit available
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

REFERENCES:

  1. McCleary, B. V. and Glennie-Holmes, M. (1985). Enzymic quantification of (1-3)(1-4)-β-D-glucan in barley and malt. J. Inst. Brew. 91, 285-295.
  2. McCleary, B. V. and Nurthen, E. J. (1986). Measurement of (1-3)(1-4)-β-D-glucan in malt, wort and beer. J. Inst. Brew.92, 168-173.
  3. McCleary, B. V. and Codd, R. (1991). Measurement of (1-3) (1-4)-β-D-glucan in barley and oats: a streamlined enzymic procedure. J. Sci. Fd. Agric. 55, 303-312.
  4. McCleary, B. V. and Mugford, D. C. (1992). Interlaboratory evaluation of β-glucan analysis methods. In “The changing role of oats in human and animal nutrition”. Proceedings of the Fourth International Oat Conference, Adelaide, Australia. Oct 19-23.

Beta葡聚糖[酵母和蘑菇]检测试剂盒

发表于由

Beta葡聚糖[酵母和蘑菇]检测试剂盒 β-Glucan Assay Kit (Yeast & Mushroom)

产品名称：Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名称：Mushroom and yeast beta-glucan assay Kit

货号： K-YBGL

型号规格：100次

优点：成本低；所有试剂配制后的稳定性〉2年；只有酶试剂盒可用；；方法简单；包含标准品

检测原理：

检测方法：比色法

检测波长：510nm；

总检测时间：100min；

检测限：样品重量的1-100%；

适用样品：酵母制备、蘑菇制备及其他原料；

方法认证：新方法

商品说明：
For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit
Colourimetric method for the determination of Yeast and 
Mushroom β-Glucan in yeast, mushroom, foodstuffs and 
other materials
Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O → 
soluble glucan
(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)
(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose
(glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
 quinoneimine + 4H2O
(amyloglucosidase)
(6) α-Glucan + H2O    →    D-glucose
Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition:        Novel method
Advantages

  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Only enzymatic kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included