The D-Fructose and D-Glucose MegaQuant™ Format test kit is suitable for the measurement and analysis of D-fructose and D-glucose in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm).

Simple colourimetric method for the determination of
D-Fructose and D-Glucose in foodstuffs, beverages and
other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(3) F-6-P ↔ G-6-P

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(diaphorase)
(5) INT + NADPH + H⁺ → NADP⁺ + INT-formazan

Kit size:  60 assays
Method: Spectrophotometric at 505 nm
Reaction time:  ~ 10 min
Detection limit:  15.4 mg/L
Application examples: 
Grape juice / must, wine, beer, fruit juices, soft drinks, milk, jam, honey, 
dietetic foods, bread, bakery products, candies, desserts, confectionery, 
ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, 
pharmaceuticals, paper and other materials (e.g. biological cultures, 
samples, etc.)
Method recognition:  Novel method

Advantages

• Novel product, patented technology
  
• Spectrophotometer / laboratory expertise not required
  
• Highly stable reagents (at least three seasons use)
  
• Very competitive price (cost per test)
  
• Very simple procedure
  
• Rapid reaction time (~ 10 min)
  
• Standard included