麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒 Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) 货号:MK-MBG4 品牌:Megazyme


麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒

英文名:Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

货号:MK-MBG4

规格:100 assays (manual) / 400 assays (auto-analyser)

商品描述

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
 
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
 
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
 
Discover more assay kits for enzyme activity measurement.
 
Content: (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or
(Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: β-Glucanase/Lichenase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: (Malt β-glucanase) 4.3 x 10-4 U/mL Or
(Lichenase) 9.1 x 10-5 U/mL
Reproducibility (%): ~ 3%
Total Assay Time: (Malt β-glucanase) ~ 20 min Or
(Lichenase) ~ 10 min 
Application examples: Crude malt extracts, industrial enzyme preparations.
Method recognition: Novel method
 
Advantages
Very cost effective 
All reagents stable for > 2 years 
Specific for endo-1,3:1,4-β-glucanase/lichenase 
Simple, convenient, rapid assay 
Well suited to automation 
Malt flour standard and lichenase standard included
 
MBG4试剂含有单一底物,即4,6-O-亚苄基-2-氯-4-硝基苯基-β-(31-β-D-纤维三糖基葡糖苷)(BCNPBG4)。亚苄基缩醛基团阻止外作用水解酶如β-葡萄糖苷酶或纤维二水解酶的任何水解作用。
混合连接的β-葡聚糖酶(内切-1,3:1,4-β-葡聚糖蛋白酶)/地衣酶(EC 3.2.1.73)特异性地从该底物中释放2-氯-4-硝基苯酚(CNP)。CNP的释放速率与样品中的β-葡聚糖酶/地衣酶活性直接相关。终止反应并在加入Tris缓冲溶液(pH=10.0)时形成酚酸酯颜色。
注意,底物不被β-葡萄糖苷酶或纤维二水解酶水解。底物可以被某些内切纤维素酶(例如木霉属)水解,但这不会导致吸光度的增加。
发现更多用于酶活性测量的检测试剂盒。
含量:(麦芽β-葡聚糖酶)100测定(手动)/400(自动分析仪)或
(Lichenase)100/200测定(手动)/330(自动分析仪)
运输温度:环境温度
储存温度:短期稳定性:2-8oC,
长期稳定性:参见单个组件标签
稳定性:在推荐的储存条件下超过2年
分析物:β-葡聚糖酶/Lichenase
测定形式:分光光度计、自动分析仪
检测方法:吸光度
波长(nm):400
信号响应:增加
检测限:(麦芽β-葡聚糖酶)4.3 x 10-4 U/mL或
(Lichenase)9.1 x 10-5 U/mL
再现性(%):~3%
总测定时间:(麦芽β-葡聚糖酶)~20分钟或
(Lichenase)~10分钟
应用实例:粗麦芽提取物、工业酶制剂。
方法识别:一种新颖的方法
优点
非常经济高效
所有试剂稳定超过2年
对内切-1,3:1,4-β-葡聚糖酶/地衣酶具有特异性
简单、方便、快速测定
非常适合自动化

包括麦芽粉标准和地衣酶标准