标签归档：异亮氨酸

4-Hydroxyisoleucine (Synonyms: 4-羟基-L-异亮氨酸; 4-​Hydroxy-​L-​isoleucine)

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白、同位素标记物，专注于信号通路和疾病研究领域。
4-Hydroxyisoleucine  (Synonyms: 4-羟基-L-异亮氨酸; 4-​Hydroxy-​L-​isoleucine) 纯度: ≥98.0%

4-Hydroxyisoleucine 是可以从胡芦巴种子中分离得到的一种具有口服活性的氨基酸。4-Hydroxyisoleucine 具有促胰岛素和抗糖尿病的特性。

4-Hydroxyisoleucine (Synonyms: 4-羟基-L-异亮氨酸; 4-​Hydroxy-​L-​isoleucine)

4-Hydroxyisoleucine Chemical Structure

CAS No. : 781658-23-9

规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥550 In-stock
1 mg ￥250 In-stock
5 mg ￥500 In-stock
10 mg ￥800 In-stock
20 mg ￥1250 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

4-Hydroxyisoleucine 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Anti-diabetic Compound Library
  • Pyroptosis Compound Library
  • Orally Active Compound Library
  • Traditional Chinese Medicine Active Compound Library
  • Plant-Sourced Natural Product Library

•同靶点产品:

•同靶点蛋白产品:

生物活性

4-Hydroxyisoleucine is an orally active amino acid that can be isolated from fenugreek seeds. 4-Hydroxyisoleucine displays insulinotropic and antidiabetic properties[1].

体外研究
(In Vitro)

4-Hydroxyisoleucine (20 μM, 6 h) 在 LPS (HY-D1056) 诱导的 RAW264.7 巨噬细胞和 3 T3-L1 脂肪细胞中降低 iRhom2、TACE、TNF-α 和 MCP-1的水平[2]。
4-Hydroxyisoleucine (20 μM, 6 h) 在 LPS (HY-D1056) 刺激的共培养系统中会增加 M1 巨噬细胞 IL-6 产物，并减少 M2 巨噬细胞 IL-10 产物[2]。
4-Hydroxyisoleucine (0-25 μM, 16 h) 浓度依赖的方式增加 L6-GLUT4myc 肌管的葡萄糖摄取和表面 GLUT4myc 水平[3]。
4-Hydroxyisoleucine (0-25 μM, 16 h) 在 L6-GLUT4myc 肌管中通过 PI-3-K/AKT 依赖机制刺激 GLUT4myc 易位[3]。

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

4-Hydroxyisoleucine 相关抗体:

Cell Viability Assay[2]

Cell Line: RAW264.7 macrophages and 3 T3-L1 adipocytes
Concentration: 0-20 μM
Incubation Time: 6 h
Result: Didn’t affect the cell viability.

Cell Migration Assay [2]

Cell Line: RAW264.7 macrophages and 3 T3-L1 adipocytes
Concentration: 20 μM
Incubation Time: 24 h
Result: Inhibited the migration of RAW264.7 macrophages in 3 T3-L1 adipocytes.

Western Blot Analysis[3]

Cell Line: L6-GLUT4myc
Concentration: 25 μM
Incubation Time: 16 h
Result: Increased the AKT (Ser-473) phosphorylation.
Didn’t effect mRNA levels of IRS-1, AKT, GSK3b, or GLUT4 in L6-GLUT4myc myotubes.

体内研究
(In Vivo)

4-Hydroxyisoleucine (50 mg/kg，口服，每日一次，8 周) 可抑制果糖喂养的代谢综合征大鼠模型中血糖的升高[1]。
4-Hydroxyisoleucine (200 mg/kg, 口服, 8 周) 改善 C57BL/6 小鼠中血脂异常，减少脂质异位积累[4]。
4-Hydroxyisoleucine (200 mg/kg, 口服, 8 周) 降低 C57BL/6 小鼠中细胞促炎因子的表达 (IL-6, PAI-1, IL-1β, NF-κB, TNF-α, and MCP-1) 和促炎 M1 巨噬细胞的比例[4]。
4-Hydroxyisoleucine (50 mg/kg, 灌胃, 每天一次, 14 天) 可将 1 型糖尿病大鼠的高血脂 (胆固醇、HDL、LDL 和甘油三酯) 和尿酸恢复至非糖尿病对照组的水平[5]。

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Fructose-fed rat[1]
Dosage: 50 mg/kg
Administration: Oral gavage (p.o.), daily, 8 weeks
Result: Decreased the levels of glucose and ALT.
Reduced 80% of fructosehe-induced AST release to 151 ± 45 U/mL.
Animal Model: Type 2 diabetic rat[1]
Dosage: 50 mg/kg
Administration: i.g., daily, 14 days
Result: Restored the level of HDL-cholesterol to levels comparable to controls.
Animal Model: Male C57BL/6 mice[4]
Dosage: 50-200 mg/kg
Administration: Oral gavage (p.o.), 8 weeks
Result: Decreases the body weights of mice in a dose-dependent manner.
Decreased blood glucose levels and fasting plasma insulin content in mice.
Decreased the expression of TLR4, inhibited the phosphorylation of JNK, and increased the production of IκB-α.
Animal Model: Type 1 diabetic rat[5]
Dosage: 50 mg/kg
Administration: i.g., daily, 14 days
Result: Improved appearance and heavy ocular vascularization.
Reduced the blood glucose from 500 mg/dl to 330 mg/dl.
Decreased the levels of lipid markers (TG, LDL and HDL) and uric acid.
Didn’t increase the level of inculin compared with untreated diabetic controls.

分子量

147.17

Formula

C6H13NO3
CAS 号

781658-23-9
性状

固体

颜色

White to off-white

中文名称

4-羟基异亮氨酸；4-羟基-L-异亮氨酸
结构分类
  • Ketones, Aldehydes, Acids
初始来源
  • 植物
  • 豆科
  • 胡卢巴
  • 植物
  • 其他科
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

H2O 中的溶解度 : 100 mg/mL (679.49 mM; 超声助溶)

DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 6.7949 mL 33.9743 mL 67.9486 mL
5 mM 1.3590 mL 6.7949 mL 13.5897 mL
10 mM 0.6795 mL 3.3974 mL 6.7949 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

=

浓度

×

体积

×

分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×

体积 (start)

V1

=

浓度 (final)

C2

×

体积 (final)

V2

In Vivo:

以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： PBS

    Solubility: 100 mg/mL (679.49 mM); 澄清溶液; 超声助溶

扫码获得
动物溶解方案

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果
工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
免费服务热线：021-50837765
E-mail：sales@jinpanbio.com
技术支持电话：021-50837765
技术支持邮箱：sales@jinpanbio.com

纯度 & 产品资料

纯度: ≥98.0%

Data Sheet (621 KB) SDS (393 KB)

COA (193 KB) HNMR (89 KB)

产品使用指南 (1538 KB)

参考文献

  • [1]. Haeri MR, et al. The effect of fenugreek 4-hydroxyisoleucine on liver function biomarkers and glucose in diabetic and fructose-fed rats. Phytother Res. 2009 Jan;23(1):61-4.  [Content Brief]

    [2]. Zhou C, et al. 4-Hydroxyisoleucine relieves inflammation through iRhom2-dependent pathway in co-cultured macrophages and adipocytes with LPS stimulation. BMC Complement Med Ther. 2020 Dec 9;20(1):373.  [Content Brief]

    [3]. Jaiswal N, et al. 4-Hydroxyisoleucine stimulates glucose uptake by increasing surface GLUT4 level in skeletal muscle cells via phosphatidylinositol-3-kinase-dependent pathway. Eur J Nutr. 2012 Oct;51(7):893-8.  [Content Brief]

    [4]. Yang J, et al. 4-Hydroxyisoleucine Alleviates Macrophage-Related Chronic Inflammation and Metabolic Syndrome in Mice Fed a High-Fat Diet. Front Pharmacol. 2021 Jan 21;11:606514.  [Content Brief]

    [5]. Haeri MR, et al. Non-insulin dependent anti-diabetic activity of (2S, 3R, 4S) 4-hydroxyisoleucine of fenugreek (Trigonella foenum graecum) in streptozotocin-induced type I diabetic rats. Phytomedicine. 2012 May 15;19(7):571-4.  [Content Brief]

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务