N4-Acetylcytidine triphosphate (Synonyms: ac4CTP)

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N4-Acetylcytidine triphosphate  (Synonyms: ac4CTP)

N4-Acetylcytidine triphosphate 可有效用作 T7 RNA 聚合酶体外催化转录的底物，并可整合到多个模板中。

N4-Acetylcytidine triphosphate (Synonyms: ac4CTP)

N4-Acetylcytidine triphosphate Chemical Structure

CAS No. : 1428903-57-4

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生物活性

N4-Acetylcytidine triphosphate is efficiently used as a substrate in T7 Polymerase-catalyzed in vitro transcription and can be incorporated into multiple templates[1].

IC50 & Target[1]

Human Endogenous Metabolite

 

体外研究
(In Vitro)

N4-Acetylcytidine triphosphate (0.1-2 mM; 24-48 h) inhibits BV2 microglia cell grwoth[1].
N4-Acetylcytidine triphosphate (0.3-1 mM; 3-6 h) activates NFκB signaling and upregulates NLRP3 expression[1].
N4-Acetylcytidine triphosphate (0.3-1 mM; 3 h) increases the expression level of HMGB1[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

N4-Acetylcytidine triphosphate 相关抗体:

Cell Viability Assay[1]

Cell Line: BV2 microglia
Concentration: 0.1-2 mM
Incubation Time: 24-48 hours
Result: Significantly inhibited BV2 microglia cell growth util cultured for 48 hours with a concentration of 0.3 mM.

Cell Viability Assay[1]

Cell Line: BV2 microglia
Concentration: 0.3 and 1 mM
Incubation Time: 3 and 6 hours
Result: Increased NLRP3 and NFκB expression at both mRNA and protein levels, activated the NFκB signaling.

Western Blot Analysis[1]

Cell Line: BV2 microglia
Concentration: 0.3 and 1 mM
Incubation Time: 3 hours
Result: Induced HMGB1 expression and it is necessary for NFkB signaling, NLRP3 expression and persistent HMGB1 expression.

分子量

525.19

Formula

C11H18N3O15P3
CAS 号

1428903-57-4
中文名称

N4-乙酰基胞苷三磷酸盐
结构分类
  • Others
初始来源
  • 内源性代谢物
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
溶解性数据
In Vitro: 

H2O 中的溶解度 : ≥ 250 mg/mL (476.02 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9041 mL 9.5204 mL 19.0407 mL
5 mM 0.3808 mL 1.9041 mL 3.8081 mL
10 mM 0.1904 mL 0.9520 mL 1.9041 mL

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* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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浓度

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分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

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体积 (start)

V1

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浓度 (final)

C2

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体积 (final)

V2

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动物溶解方案

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

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每只动物的给药体积

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工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
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纯度 & 产品资料
Data Sheet (593 KB) 产品使用指南 (1538 KB)

参考文献

  • [1]. Duan J, et al. N4-acetylcytidine is required for sustained NLRP3 inflammasome activation via HMGB1 pathway in microglia. Cell Signal. 2019 Mar 7;58:44-52.  [Content Brief]

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