Lupenone

发表于由

上海金畔生物科技有限公司为生命科学和医药研发人员提供生物活性分子抑制剂、激动剂、特异性抑制剂、化合物库、重组蛋白、同位素标记物，专注于信号通路和疾病研究领域。
Lupenone  纯度: ≥98.0%

Lupenone 是一种具有口服活性的可从 Musa basjoo 中分离出的 lupine 型三萜类化合物。Lupenone Lupenone 可通过 PI3K/Akt/mTOR 及 NF-κB 信号通路发挥作用。Lupenone 具有抗炎、抗病毒、抗糖尿病和抗癌活性。

Lupenone

Lupenone Chemical Structure

CAS No. : 1617-70-5

规格 价格 是否有货 数量
1 mg ￥700 In-stock
5 mg ￥1700 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Lupenone 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Immunology/Inflammation Compound Library
  • Kinase Inhibitor Library
  • NF-κB Signaling Compound Library
  • PI3K/Akt/mTOR Compound Library
  • Stem Cell Signaling Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Autophagy Compound Library
  • Anti-Aging Compound Library
  • Antioxidant Compound Library
  • Anti-diabetic Compound Library
  • Oxygen Sensing Compound Library
  • Medicine Food Homology Compound Library
  • Terpenoids Library
  • Glycolysis Compound Library
  • Pyroptosis Compound Library
  • Cytoskeleton Compound Library
  • Orally Active Compound Library
  • Glutamine Metabolism Compound Library
  • Traditional Chinese Medicine Active Compound Library
  • Anti-Breast Cancer Compound Library
  • Anti-Pancreatic Cancer Compound Library
  • Antiparasitic Compound library
  • Anti-Cancer Metabolism Compound Library
  • Angiogenesis-Related Compound Library
  • Transcription Factor-Targeted Library
  • Glucose Metabolism Compound Library
  • Anti-Cancer Natural Product Library
  • Anti-inflammatory Traditional Chinese Medicine Active Compound Library
  • Plant-Sourced Natural Product Library
  • Human Metabolite Library
  • Pain-Related Compound Library
  • Membrane Protein-targeted Compound Library
  • Multi-Target Compound Library
  • Radioprotector Library

•同靶点产品:

•同靶点蛋白产品:

生物活性

Lupenone is an orally active lupine-type triterpenoid that can be isolated from Musa basjoo. Lupenone Lupenone plays a role through the PI3K/Akt/mTOR and NF-κB signaling pathways. Lupenone has anti-inflammatory, antiviral, antidiabetic and anticancer activities[1][2][3].

IC50 & Target

Trypanosoma

 

体外研究
(In Vitro)

Lupenone (40 μM, 1 h) 通过 PI3K/Akt/mTOR 信号通路保护神经母细胞瘤 SH-SY5y 细胞免受甲基苯丙胺诱导的凋亡细胞死亡[1]。

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Lupenone 相关抗体:

Cell Viability Assay[1]

Cell Line: SH-SY5y
Concentration: 5, 10. 20, 40 μM
Incubation Time: 24 h
Result: Did not cause significant cell death at different concentrations.

Apoptosis Analysis[1]

Cell Line: SH-SY5y
Concentration: 40 μM
Incubation Time: 1 h
Result: Reduced the population of annexinV/PI-positive cells (early apoptotic cells) and annexinV positive cells (total apoptotic cells).

Western Blot Analysis[1]

Cell Line: SH-SY5y
Concentration: 40 μM
Incubation Time: 1 h
Result: Increased the expression of anti-apoptotic proteins, including bcl-2 and caspases such as caspase 3, caspase 7, and caspase 8.
Recuperate the phosphorylation level of PI3K and Akt.
Inhibited the translocation of p65 from the cytosol to the nucleus, IκBα degradation, and IκBα phosphorylation.

体内研究
(In Vivo)

Lupenone (6, 12, 24 mg/kg/day, 灌胃, 连续 6 周) 通过调节 NF-κB 通路介导的炎症和 TGF-β1/Smad/ CTGF 相关纤维化改善 2 型糖尿病肾病[2]。

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Spontaneous type 2 diabetic nephropathy db/db mouse models[1]
Dosage: 6, 12, 24 mg/kg
Administration: i.g.
Result: Maintained the fasting blood glucose.
Reduced glycosylated hemoglobin, insulin, and 24 h proteinuria levels.
Regulated changes in biochemical indicators

分子量

424.70

Formula

C30H48O
CAS 号

1617-70-5
性状

固体

颜色

White to off-white

结构分类
  • Terpenoids
  • Triterpenes
初始来源
  • 植物
  • 豆科
  • Astragalus oleifolius DC.
  • 植物
  • 芭蕉科
  • 芭蕉
  • 植物
  • 菊科
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

Ethanol 中的溶解度 : 10 mg/mL (23.55 mM; 超声助溶 (<60°C))

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3546 mL 11.7730 mL 23.5460 mL
5 mM 0.4709 mL 2.3546 mL 4.7092 mL
10 mM 0.2355 mL 1.1773 mL 2.3546 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

=

浓度

×

体积

×

分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×

体积 (start)

V1

=

浓度 (final)

C2

×

体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (2.35 mM); 澄清溶液

    此方案可获得 ≥ 1 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 10.0 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。

  • 方案 二

    请依序添加每种溶剂： 10% EtOH    90% Corn Oil

    Solubility: ≥ 1 mg/mL (2.35 mM); 澄清溶液

    此方案可获得 ≥ 1 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。

    以 1 mL 工作液为例，取 100 μL 10.0 mg/mL 的澄清 EtOH 储备液加到 900 μL 玉米油中，混合均匀。

扫码获得
动物溶解方案

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果
工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: ≥98.0%

Data Sheet (630 KB) SDS (251 KB)

COA (269 KB) HNMR (194 KB)

产品使用指南 (1538 KB)

参考文献

  • [1]. Lee HS, et al. Lupenone Protects Neuroblastoma SH-SY5y Cells Against Methamphetamine-Induced Apoptotic Cell Death via PI3K/Akt/mTOR Signaling Pathway. Int J Mol Sci. 2020 Feb 27;21(5):1617.  [Content Brief]

    [2]. Wu H, et al. Lupenone improves type 2 diabetic nephropathy by regulating NF-κB pathway-mediated inflammation and TGF-β1/Smad/CTGF-associated fibrosis. Phytomedicine. 2023 Sep;118:154959.  [Content Brief]

    [3]. Mukerjee N, et al. Dynamics of natural product Lupenone as a potential fusion inhibitor against the spike complex of novel Semliki Forest Virus. PLoS One. 2022 Feb 25;17(2):e0263853.  [Content Brief]

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务