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Iberin (Synonyms: NSC 321801) 纯度: 98.0%
Iberin (NSC 321801) 是萝卜硫素的亚砜类似物,是天然存在的异硫氰酸酯家族的成员。抑制 HL60 细胞的 IC50 值为 2.3 μM。Iberin 诱导凋亡 (apoptosis)。
Iberin Chemical Structure
CAS No. : 505-44-2
规格 | 价格 | 是否有货 | 数量 |
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1 mg (61.25 mM * 100 μL in Ethanol) | ¥500 | In-stock | |
5 mg (61.25 mM * 500 μL in Ethanol) | ¥1100 | In-stock | |
10 mg (61.25 mM * 1 mL in Ethanol) | ¥1800 | In-stock |
* Please select Quantity before adding items.
生物活性 |
Iberin (NSC 321801), a sulfoxide analogue of sulforaphane, is a naturally occurring member of isothiocyanate family. Iberin inhibits cell survival with an IC50 of 2.3 μM in HL60 cell. Iberin induces apoptosis. |
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IC50 & Target |
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体外研究 (In Vitro) |
Iberin inhibits the growth of neuroblastoma cells in a dose- and time-dependent manner. The iberin-induced cell cycle arrest in neuroblastoma cells is associated with inhibition of expression of cyclin-dependent kinase Cdk2, Cdk4, and Cdk6 proteins. There is an increase in apoptotic cell death in iberin treated cells as compared with control cells. The iberin-induced apoptosis is found to be associated with activation of caspase-9, caspase-3, and PARP[2]. Iberin inhibits growth of human glioblastoma cells in cell proliferation assays, enhances cytotoxicity, and induces apoptosis by activation of caspase-3 and caspase-9[3]. Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only. |
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体内研究 (In Vivo) |
Iberin is tested in an in vivo foreign-body infection mouse model, and the results show no significantly difference in bacterial clearance between treated and nontreated miced[4]. Iberin increases tissue levels of the phase II detoxification enzymes quinone reductase and glutathione S-transferase in a variety of rat tissues[5]. Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
163.26 |
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Formula |
C5H9NOS2 |
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CAS 号 |
505-44-2 |
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性状 |
液体 |
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颜色 |
Colorless to light yellow |
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结构分类 |
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初始来源 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
Solution, -20°C, 2 years |
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溶解性数据 |
In Vitro:
DMSO 中的溶解度 : 100 mg/mL (612.52 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO) Ethanol 中的溶解度 : 10 mg/mL (61.25 mM; 超声助溶)
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2
In Vivo:
请根据您的 实验动物和给药方式 选择适当的溶解方案。 以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
动物溶解方案计算器
请输入动物实验的基本信息:
给药剂量 mg/kg 动物的平均体重 g 每只动物的给药体积 μL 动物数量 只 由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO + %
%
Tween-80 + %
Saline 如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。 方案所需 助溶剂 包括:DMSO, ,均可在 Shanghai Jinpan Biotech Co Ltd 网站选购。 ,Tween 80,均可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL DMSO(母液浓度为 mg/mL)。
免费服务热线:021-50837765
E-mail:sales@jinpanbio.com 技术支持电话:021-50837765 技术支持邮箱:sales@jinpanbio.com 动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL 。 μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水。
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
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纯度 & 产品资料 |
纯度: 98.0%
Data Sheet (620 KB) SDS (251 KB)
COA (164 KB) 产品使用指南 (1538 KB) |
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参考文献 |
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Cell Assay [2] |
Cells are plated at a density of 1×105 cells/well in microtiter plates and treated with different concentrations of iberin (1, 2.5, 10 and 25 μM). Then 20 μL of 5 mg/mL MTT in PBS, is added to each well and allowed to incubate for a further 4 h. After 4 h of incubation, 100 μL of DMSO is added to each well to dissolve the formazan crystals. Absorbance values at 550 nm are measured with a microplate reader[2]. Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only. |
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Animal Administration [4][5] |
Rats: Groups of five rats are dosed by oral intubation with the test compounds, as solutions in soybean oil, each day for 5 days. This doses used are 4.0 mg/kg/day for AITC, 5.9 mg/kg/day for iberverin, 6.5 mg/kg/day for iberin, 6.4 mg/kg/day for erucin, 7.1 mg/kg/day for sulforaphane, and 7.2 mg/kg/day for cheirolin. The volume of solution administered is 2 mL/kg in all cases. Ten control rats are dosed with soybean oil alone[5]. Mice: Iberin is diluted in 96% ethanol to a concentration of 32 mg/mL followed by a 40x dilution in 0.9% NaCl. The mice are injected with 0.2 mL of the final solution, corresponding to 8 μg/g of body weight. The placebo group is injected with a 2.4% ethanol solution (96% ethanol–0.9% NaCl) corresponding to the amount of ethanol that the iberin-treated group received. Mice are treated every 12 h from day 2 preinsertion to day 2 postinsertion, and treatment is continued until 12 h before the mice are euthanized[4]. Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
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