Lacto-N-fucopentaose I (Synonyms: LNFP I)

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Lacto-N-fucopentaose I (Synonyms: LNFP I)

Lacto-N-fucopentaose I (LNFPI) 是一种人母乳寡糖 (HMO)，具有抗病毒和抗菌活性。Lacto-N-fucopentaose I 通过减少衣壳蛋白 VP1 来阻断病毒吸附，增加 CDK2、减少 cyclin E 恢复细胞周期 S 期的阻滞。Lacto-N-fucopentaose I 抑制病毒感染的细胞凋亡 (apoptosis)。Lacto-N-fucopentaose I 也抑制 ROS 的产生。Lacto-N-fucopentaose I 还可以调节肠道菌群影响免疫系统发育。

Lacto-N-fucopentaose I Chemical Structure

CAS No. : 7578-25-8

规格	价格	是否有货
1 mg	￥3795	询问价格 & 货期
5 mg	￥8350	询问价格 & 货期

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Lacto-N-fucopentaose I 相关产品

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生物活性

Lacto-N-fucopentaose I (LNFPI) is a human milk oligosaccharide (HMO), possessing antiviral and antibacterial activity. Lacto-N-fucopentaose I can reduce capsid protein VP1 to block virus adsorption, promote CDK2 and reduce cyclin E to recover cell cycle S phase block. Lacto-N-fucopentaose I inhibits ROS production and apoptosis in virus-infected cells. Lacto-N-fucopentaose I can also regulate intestinal microbiota to affect immune system development^[1].

IC₅₀ & Target

Human Endogenous Metabolite

CDK2/cyclinE

体外研究
(In Vitro)

Lacto-N-fucopentaose I (25-3200 μg/mL;48 h) exhibits certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL^[1].
Lacto-N-fucopentaose I (25-1600 μg/mL; 14-18 h) can protect EV71-infected RD cells from death^[1].
Lacto-N-fucopentaose I (100-400 μg/mL; 16 h) decreases markedly mRNA levels of VP1 and ROS production in EV71-infected RD cells at 400 μg/mL; leads to the recovery of EV71-induced S phase arrest in RD cells^[1].
Lacto-N-fucopentaose I (100 and 200 μg/mL; 3 days) inhibits cell apoptosis in Caenorhabditis elegans; significantly decreases the levels of Egl-1, Ced-3 and Ced-4^[1].
Lacto-N-fucopentaose I can reduce the abundance of Sphingobacterium, Stenotrophomonas and Achromobacter; can increase the abundance of Micromonospora, Vibrio, Acidibacter, Gaiella, Devosia, Steroidobacter, Variibacter, Dactylosporangium, RB41, Pir4_lineage, Pirellula, Haliangium, Roseiflexus, Pedomicrobium, and Bradyrhizobium.

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Lacto-N-fucopentaose I 相关抗体:

Cell Viability Assay^[1]

Cell Line:	RD cells
Concentration:	25, 50, 100, 200, 400, 800, 1600 and 3200 μg/mL
Incubation Time:	48 h
Result:	Exhibited certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL.

RT-PCR^[1]

Cell Line:	RD cells (infected with EV71)
Concentration:	100, 200 and 400 μg/mL
Incubation Time:	16 h
Result:	Decreased markedly mRNA levels of VP1 only at 400 μg/mL.

Apoptosis Analysis^[1]

Cell Line:	RD cells (infected with EV71)
Concentration:	100, 200 and 400 μg/mL
Incubation Time:	16 h
Result:	Decreased the rate of cells in early apoptosis to 10.9% ± 1.26% at 400 μg/mL, while the untreated EV71 group was 27.7% ± 2.13%. Significantly inhibited the activity of caspase-3, caspase-8 and caspase-9. Recovered the decreased mRNA expression of PAPR, NF-κB and Bcl-2 and properly regulated Bad and Fas into their normal levels.

Cell Cycle Analysis^[1]

Cell Line:	RD cells (infected with EV71)
Concentration:	100, 200 and 400 μg/mL
Incubation Time:	12 h
Result:	Rescued EV71-induced S phase arrest, which promoted the transition of the G1 phase to the S phase.

分子量

853.77

Formula

C₃₂H₅₅NO₂₅

CAS 号

7578-25-8

性状

固体

颜色

White to off-white

结构分类

Saccharides
Polysaccharides

Others

初始来源

动物

human milk

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

纯度 & 产品资料

纯度: ≥90.0%

选择批次：

Data Sheet (546 KB) SDS (252 KB)

COA (278 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Gao X, et al. Fucosylated oligosaccharide Lacto-N-fucopentaose I ameliorates enterovirus 71 infection by inhibiting apoptosis. Food Chem X. 2022 Feb 4;13:100244. [Content Brief]

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