热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions) 货号:422050 品牌:Sygnis


热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions)

货号:422050

规格:50reactions

报价:5700.00

商品描述

 SunScipt™ Reverse Transcriptase

SunScipt™ 逆转录酶是一种新颖的高热稳定性逆转录酶,可以大大提升cDNA的合成效率。SunScipt™ 逆转录酶有两种,分别为RNase H+与RNase H-。

RNase H+:有很高的热稳定性,在85℃情况下仍具有活性,具有RNA与DNA依赖性的聚合酶活性和RNase H活性。

RNase H-:有很高的热稳定性,没有RNase H活性,用于具有复杂二级结构的长链RNA的逆转录,cDNA产量很高。

产品优势:

·高热稳定性,85℃仍可反应;

·cDNA一链合成效率高;

·可用于哺乳动物长链mRNA合成;

·好的灵敏度(起始RNA量低至皮克级);

·高重复率,结果可信度高。

逆转录反应原理

热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions)  货号:422050 品牌:Sygnis

 

实验比较

热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions)  货号:422050 品牌:Sygnis

热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions)  货号:422050 品牌:Sygnis

热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions)  货号:422050 品牌:Sygnis

热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions)  货号:422050 品牌:Sygnis

热稳定性逆转录酶 SunScript™ RT  RNaseH-(50reactions)  货号:422050 品牌:Sygnis

 
unScript™ Reverse Transcriptase ( RNaseH- ) is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity based on the well characterized HIV-1 RT. SunScript can completely synthesise cDNA of up to 11 Kb in length in only 5 minutes.

Additional Information

Shelf Life 1 Year from receipt
Storage +4C

Supply

SunScript™ Reverse Transcriptase RNaseH- is supplied in a box, with sufficient amount for either 10, 50 or 200 reactions. The box also includes 5X Reaction Buffer and 0.1 M DTT. Contents are provided in clear, screw-cap tubes with colour-coded lids as referenced at “Contents” at the end of this document.

Shipping and Storage

This product is shipped in dry ice. Upon receipt, it should be stored immediately at -20°C in a non-frost-free (constant temperature) freezer. If stored correctly, the product can be kept for at least six months after shipping without displaying any reduction in performance. For longer periods of time, store the kit at -80°C.

Technical specifications

SunScript™ Reverse Transcriptase RNaseH- has been tested to be free of endonucleases, exonucleases and ribonucleases. The purity of the enzyme is guaranteed to be higher than 95%. The enzyme and reagents have been tested for amplification of 16 Kb first strand cDNA for 60 min at 65°C.

Quality Control

Each batch of SunScript™ Reverse Transcriptase RNaseH- is tested against predetermined specifications to ensure consistent product quality.

Intended use

SunScript™ Reverse Transcriptase RNaseH- is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.

Patent

Patent protection pending for SunScript™ Reverse Transcriptase RNaseH- and methods associated with its use.

Safety information:

Safety data sheets are available for download from www.sygnis.com. This material may contain substances or activities that are harmful to human health. It should not be ingested, inhaled, or brought into contact with skin, and handled with appropriate care in accordance with the principles of good laboratory practices. DTT is irritating (to eyes, respiratory system and skin), and harmful if swallowed. Risk and safety phrases: (R22, R36/37/38).

Contents

Description Cap color 10 reactions 50 reactions 200 reactions
SunScript™ RT RNaseH- Orange 1 x 15 µl 1 x 75 µl 1 x 300 µl
5X Reaction Buffer Yellow 1 x 40 µl 1 x 200 µl 1 x 800 µl
DTT White 1 x 20 µl 1 x 100 µl 1 x 400 µl