D-葡萄糖醛酸和D-半乳糖醛酸检测试剂盒 D-Glucuronic/D-Galacturonic Acid Assay Kit 货号:MK-URONIC 品牌:Megazyme


D-葡萄糖醛酸和D-半乳糖醛酸检测试剂盒

英文名:D-Glucuronic/D-Galacturonic Acid Assay Kit

货号:MK-URONIC

规格:100 assays (manual) / 1000 assays (microplate)

商品描述

The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of D-Glucuronic Acid or
D-Galacturonic Acid in hydrolysates of plant material and
polysaccharides and other materials

Principle:
(Uronate dehydrogenase; UDH)
(1) D-Glucuronic acid + NAD+ + H2O → D-glucarate + NADH + H+

(Uronate dehydrogenase; UDH)
(2) D-Galacturonic acid + NAD+ + H2O → D-galactarate + NADH + H+

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1000 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min at 25°C or ~ 5 min at 37°C
Detection limit: ~ 17 mg/L
Application examples:
Hydrolysates of plant material and polysaccharides and other
materials
Method recognition: Novel method

Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only test kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats