分析物意义： 常见食品组分和添加剂 

Megazyme检测试剂盒优点：适用于手工和自动分析仪进行检测。重组的柠檬酸裂解酶-20摄氏度的稳定性>6个月。试剂稳定 

A flexible and simple method for the rapid and reliable measurement of citric acid (citrate) in foods, beverages and other materials. Content: 72 assays per kit

UV-method for the determination of Citric Acid in foods,
beverages and other materials

Principle:
(citrate lyase)
(1) Citrate → oxaloacetate + acetate

(L-malate dehydrogenase)
(2) Oxaloacetate + NADH + H⁺ → L-malate + NAD⁺

(D-lactate dehydrogenase)
(3) Pyruvate + NADH + H⁺ → D-lactate + NAD⁺

Kit size:                             72 assays (manual) / 720 (microplate) 
                                          / 840 (auto-analyser)
Method:                             Spectrophotometric at 340 nm
Reaction time:                   ~ 5 min
Detection limit:                  0.921 mg/L
Application examples: 
Grape juice, wine, beer, fruit juices, soft drinks, tea, dairy products 
(e.g. cheese), meat, processed meat, vegetable and fruit products, 
bakery products, paper, pharmaceuticals, cosmetics and other 
materials (e.g. biological cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by MEBAK, OIV, 
EU, ISO2963, AOAC and IFU22
(Note: If the enzyme oxaloacetate decarboxylase is present in the sample, some 
of the oxaloacetate product is converted to pyruvate. Therefore, to ensure citric
acid is measured quantitatively, D-lactate dehydrogenase (D-LDH) is employed
to efficiently convert any pyruvate produced into D-lactate and NAD+).

Advantages

• Reconstituted citrate lyase stable for 4 weeks at 4°C / 6 months at -20°C
  
• Buffer / cofactor / enzyme tablets for efficient use of kit components
  
• PVP incorporated to prevent tannin inhibition
  
• Very competitive price (cost per test)
  
• Mega-Calc™software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Extended cofactors stability
  
• Suitable for manual, microplate and auto-analyser formats